Circulating insulin concentrations and lipogenesis in inbred lines of mice differing in fatness
نویسندگان
چکیده
( a ) Microsomes were incubated with [ 'Tlserine (2 mM) in 40 mM-Hepes buffer, pH 7.4, containing 0.25 M-sucrose and 1 mM-CaCI,. After each incubation time the microsomes were treated with TNBS and the lipids extracted and analysed (Hutson & Hig ins, 1985). ( h ) Hepatocytes were incubated with 2 mwj"C7serine in Krebs-Henseleit buffer. The cells and medium were separated by centrifugation and the lipids of each extracted and analysed. Only the data for incorporation of serine into secreted phospholipid are shown. TNP, trinitrophenol.
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